Journal: Journal of Tissue Engineering

Article Title: Cell secretomes for wound healing and tissue regeneration: Next generation acellular based tissue engineered products

doi: 10.1177/20417314221114273

Figure Lengend Snippet: List of major growth factors and cytokines involve in wound healing.

Article Snippet: • Keratinocyte-conditioned medium (KCM) , • Keratinocytes serum free medium supplemented with bovine pituitary extract and epidermal growth factor. • Keratinocytes medium (CnT basal medium1 with supplements A, B, C; CELLnTEC) , • Incubated for 24 h and 48 h, filtered and stored at −20°C until use , • Collagen type-I • β-actin • Pro-α1(I) , • Regulate ECM collagen type I. • Promote viability of dermal fibroblast. • However, no effect on the fibroblast expansion , Ghaffari et al., Bukowska et al. .

Techniques: Migration

Journal: Journal of Tissue Engineering

Article Title: Cell secretomes for wound healing and tissue regeneration: Next generation acellular based tissue engineered products

doi: 10.1177/20417314221114273

Figure Lengend Snippet: Tissue-engineered skin substitutes with living cells.

Article Snippet: • Keratinocyte-conditioned medium (KCM) , • Keratinocytes serum free medium supplemented with bovine pituitary extract and epidermal growth factor. • Keratinocytes medium (CnT basal medium1 with supplements A, B, C; CELLnTEC) , • Incubated for 24 h and 48 h, filtered and stored at −20°C until use , • Collagen type-I • β-actin • Pro-α1(I) , • Regulate ECM collagen type I. • Promote viability of dermal fibroblast. • However, no effect on the fibroblast expansion , Ghaffari et al., Bukowska et al. .

Techniques: Membrane, Cell Culture, Suspension

Journal: Journal of Tissue Engineering

Article Title: Cell secretomes for wound healing and tissue regeneration: Next generation acellular based tissue engineered products

doi: 10.1177/20417314221114273

Figure Lengend Snippet: Secretomes derived from different cell sources that are used in wound healing.

Article Snippet: • Keratinocyte-conditioned medium (KCM) , • Keratinocytes serum free medium supplemented with bovine pituitary extract and epidermal growth factor. • Keratinocytes medium (CnT basal medium1 with supplements A, B, C; CELLnTEC) , • Incubated for 24 h and 48 h, filtered and stored at −20°C until use , • Collagen type-I • β-actin • Pro-α1(I) , • Regulate ECM collagen type I. • Promote viability of dermal fibroblast. • However, no effect on the fibroblast expansion , Ghaffari et al., Bukowska et al. .

Techniques: Derivative Assay, Transferring, Incubation, Migration, In Vitro, Filtration, Cell Culture

Journal: Journal of Tissue Engineering

Article Title: Cell secretomes for wound healing and tissue regeneration: Next generation acellular based tissue engineered products

doi: 10.1177/20417314221114273

Figure Lengend Snippet: Exosomes and important cells involved in diabetic wound healing. Exosomes can promote endothelial cell function recovery and angiogenesis through activating the Erk1/2signaling pathway or activation of eNOS/AKT/ERK/P-38 signaling pathways, inhibition of AP-1/ROS/NLRP3/ASC/Caspase-1/IL-1β, and increasing the secretion of VEGF, IGF-1, and FGF. Exosomes inhibit inflammation by promoting phenotypic changes of macrophages by activating the PTEN/Akt signaling pathway. Exosomes promote proliferation and migration of fibroblasts by activation of PI3K/Akt pathways or activating the Rho-YAP signaling pathway exosomes promote the proliferation and migration of keratinocytes. The figure is reprinted (adapted) with permission from reference under the CC BY-NC-ND license ( http://creativecommons.org/licenses/by-nc-nd/4.0/ ).

Article Snippet: • Keratinocyte-conditioned medium (KCM) , • Keratinocytes serum free medium supplemented with bovine pituitary extract and epidermal growth factor. • Keratinocytes medium (CnT basal medium1 with supplements A, B, C; CELLnTEC) , • Incubated for 24 h and 48 h, filtered and stored at −20°C until use , • Collagen type-I • β-actin • Pro-α1(I) , • Regulate ECM collagen type I. • Promote viability of dermal fibroblast. • However, no effect on the fibroblast expansion , Ghaffari et al., Bukowska et al. .

Techniques: Cell Function Assay, Activation Assay, Protein-Protein interactions, Inhibition, Migration